Place brine at zero temperature. Link enzyme (E) with the target material (S) at the active site of the enzyme. The temperature of the medium in which the amylase enzyme works is reduced to zero

What happens in each of the following cases:
1- Put the brine at zero temperature.
- The saline solution does not freeze.
2- The enzyme (E) is associated with the target substance (S) at the active site of the enzyme.
- A complex called the Enzyme Interferon and the Target Material (ES) is formed, the ES is dissolved and the reaction products are formed and the enzyme E is released.
3- Low temperature of the medium in which the amylase enzyme works to zero.
- Amylase activity stops.
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