bacteria need dark field to detect - Autoclave sterilize in temp

- bacteria need dark field to detect:

• A- T.pallidium.

- Autoclave sterilize in temp:

• B- 121ْc-20min.

Here's a breakdown of your statements:

- Bacteria need dark field to detect:

T.pallidium: This is correct.

Treponema pallidum, the bacterium that causes syphilis, is notoriously difficult to detect due to its small size and lack of a cell wall. Dark field microscopy is a specialized technique that uses intense light and a dark background to illuminate the organism, making it visible.

Autoclave sterilize in temp:

- B- 121°C-20min:

This is also correct. Autoclaving is a common sterilization method that uses high pressure and steam to kill all microorganisms. The standard sterilization cycle for most materials is 121°C (250°F) for 20 minutes. This temperature and time combination is effective in killing all bacterial spores, which are the most resistant form of bacteria.

Here are some additional details that might be helpful:

• Dark field microscopy.
• Autoclave.
• Treponema pallidum.