Fish suffer from many diseases caused by a wide range of bacteria, fungi and viruses such as diseases Vibrosis, ich and lymphocystis. Because symptoms of these diseases are not clearly present at the beginning of the infection, laboratory diagnosis has become a necessity.
The diagnosis in these cases is either by isolating the organism causing the disease and identification, a difficult issue requiring specialized laboratories at a high level of processing and laboratory and human capabilities trained, or diagnosis by confirming the presence of antibodies that consist of attacking the strange object and try to rid the body of it And the amount of radiation or enzymatic analysis.
Due to the difficulty of using the above methods, scientists have used genetic engineering methods to detect microorganisms directly in samples without resorting to isolation. This is done by estimating the sequence of bases in the DNA, which is known as Polymerase Chain Reaction (PCR) reactions and analysis of cells' DNA by Molecular probes, which can detect the genetic sequence of the pathogen.
The importance of PCR testing and DNA testing is to detect the least amount of the pathogen. Thus, the infection can be diagnosed at the onset of the disease and is an important step in the early diagnosis of infection before the onset of symptoms.
This method has been used to diagnose Furunculosis, one of the oldest fish diseases in the world caused by Aeromonas salmonicida bacteria.
The diagnosis in these cases is either by isolating the organism causing the disease and identification, a difficult issue requiring specialized laboratories at a high level of processing and laboratory and human capabilities trained, or diagnosis by confirming the presence of antibodies that consist of attacking the strange object and try to rid the body of it And the amount of radiation or enzymatic analysis.
Due to the difficulty of using the above methods, scientists have used genetic engineering methods to detect microorganisms directly in samples without resorting to isolation. This is done by estimating the sequence of bases in the DNA, which is known as Polymerase Chain Reaction (PCR) reactions and analysis of cells' DNA by Molecular probes, which can detect the genetic sequence of the pathogen.
The importance of PCR testing and DNA testing is to detect the least amount of the pathogen. Thus, the infection can be diagnosed at the onset of the disease and is an important step in the early diagnosis of infection before the onset of symptoms.
This method has been used to diagnose Furunculosis, one of the oldest fish diseases in the world caused by Aeromonas salmonicida bacteria.