The washing is must in all heterogenous ELISA techniques because?
a. It remove the excess binding
b. Increase the specificity
c. Increase the sensitivity.
(b) In ELISA, the first washing is used to remove the unbound (free) sample antigen. The second washing removes unreacted free label (not excess binding in either of the 2 washings)
If washing is not complete, this will ↑false high → ↓ specificity.
If the question comes as It avoids excess binding, then this will be the choice.
a. It remove the excess binding
b. Increase the specificity
c. Increase the sensitivity.
(b) In ELISA, the first washing is used to remove the unbound (free) sample antigen. The second washing removes unreacted free label (not excess binding in either of the 2 washings)
If washing is not complete, this will ↑false high → ↓ specificity.
If the question comes as It avoids excess binding, then this will be the choice.