A female client with an indwelling urinary catheter is suspected of having a urinary tract infection.. wiping the self-sealing aspiration port with antiseptic solution and aspirating urine with a sterile needle

A female client with an indwelling urinary catheter is suspected of having a urinary tract infection. Nurse Angel should collect a urine specimen for culture and sensitivity by:
a. disconnecting the tubing from the urinary catheter and letting the urine flow into a sterile container.
b. wiping the self-sealing aspiration port with antiseptic solution and aspirating urine with a sterile needle.
c. draining urine from the drainage bag into a sterile container.
d. clamping the tubing for 60 minutes and inserting a sterile needle into the tubing above the clamp to aspirate urine.

Answer B.
Most catheters have a self-sealing port for obtaining a urine specimen. Antiseptic solution is used to reduce the risk of introducing microorganisms into the catheter. Tubing shouldn’t be disconnected from the urinary catheter. Any break in the closed urine drainage system may allow the entry of microorganisms. Urine in urine drainage bags may not be fresh and may contain bacteria, giving false test results. When there is no urine in the tubing, the catheter may be clamped for no more than 30 minutes to allow urine to collect.