Pathogens were implanted on the implantation media (maconucleic glue, blood glue, glucal glue) by the planning process on each of the previously mentioned circles. The bacteria were isolated based on the shape and color of the colony and the type of decomposition it produced on the center of the blood (Akar blood) and purification on the center (Nutrient agar) or (chocolate agar) available in the laboratory and thus obtained colonies pure purity was verified by a swipe on the slide, It was dyed with Gram (s) Stain and was examined with an oily lens through a microscope.