Histological sections:
It is necessary to make sections of the body with a thickness suitable to know the status of different organs in the body and its composition, these sections fall within different levels, suffice here to mention only three types:
1- Cross-Transverse sections (C.S, T.S, XS). The cutting level is vertical on the longitudinal axis of the body.
2- Longitudinal sections (L.S). The level of the pieces is parallel to or parallel to the longitudinal axis of the body.
3- Perpendicular sections (P.S.) Where the level of cutting vertically on the surface of the body to be taken sections.
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Histological sections of different tissues affected by the WSSV. to. Gill lamellae with multiple inclusion bodies, hypertrophied cells and some fragmented nuclei are observed (arrow). b. Lick her gill presenting a centronuclear inclusion body, there is a clear halo between the inclusion body and the nuclear membrane, easily confused with the IHHNV virus. c. Antenal gland in a heavily infected animal, most cells have inclusion bodies in hypertrophied nuclei. d. Stomach epithelium, section subjected to in situ hybridization with specific probes for the virus. Dark nuclei have a positive signal to the virus. and. Section of hepatopancreas, subjected to in situ hybridization, this organ has no affinity for the virus, in severe cases of infection, positive cells are observed in the intertubular spaces. Epithelial cells are not affected. F. Hepatopancreas capsule, hypertrophied nuclei are observed, with two inclusion bodies.
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Os Histological cuts are the main instrument for or study of anatomical and histological characteristics of two vegetais. To carry out the need for the sheet, brush and plant material that will be used for visualization, other than all or laboratory equipment: optical microscopy, petri dishes, sheets and laminulas, hypochlorite and corant (fuxin, lugol, among others)
Or procedure, the objective of which is to make cuts of escolhidas or thinner structures, transparent possível to allow observation of all the tenses that they form.
The main cuts are:
-Transversal:
Horizontal and total cut. Deep or enough to allow observation of two internal tecidos.
- Longitudinal:
Vertical and total cut made in the middle of vertical direction. It allows visualization of internal tecidos.
Paradermal:
Horizontal and superficial cut, allowing for observation of external tecidosis.
Or procedure for the realization of six cuts and faith in six stages:
- Initially performed or cut:
É aconselhado se fazer replicas and escort you more transparent
- Deposit or cut in petri dish with water:
Isso lets you know that the court doesn't desidrate
Deposit or cut in hypochlorite for 2 min. => Assim a clean cut. Apos must be withdrawn or excessive in water.
- Cook or cut for 2 min:
This stage is carried out with a specific corant, seja fuxin (for cores lignin) seja lugol (for cores amido). Apos must be deposited or cut to water to throw or excess of corante.
Deposit or court na lâmina;
Cover or cut with the laminula:
in position of 45º.
Após this procedure or cut is soon to be observed not microscopic optical.
It is necessary to make sections of the body with a thickness suitable to know the status of different organs in the body and its composition, these sections fall within different levels, suffice here to mention only three types:
2- Longitudinal sections (L.S). The level of the pieces is parallel to or parallel to the longitudinal axis of the body.
3- Perpendicular sections (P.S.) Where the level of cutting vertically on the surface of the body to be taken sections.
------------------------
Histological sections of different tissues affected by the WSSV. to. Gill lamellae with multiple inclusion bodies, hypertrophied cells and some fragmented nuclei are observed (arrow). b. Lick her gill presenting a centronuclear inclusion body, there is a clear halo between the inclusion body and the nuclear membrane, easily confused with the IHHNV virus. c. Antenal gland in a heavily infected animal, most cells have inclusion bodies in hypertrophied nuclei. d. Stomach epithelium, section subjected to in situ hybridization with specific probes for the virus. Dark nuclei have a positive signal to the virus. and. Section of hepatopancreas, subjected to in situ hybridization, this organ has no affinity for the virus, in severe cases of infection, positive cells are observed in the intertubular spaces. Epithelial cells are not affected. F. Hepatopancreas capsule, hypertrophied nuclei are observed, with two inclusion bodies.
-----------------------------
Os Histological cuts are the main instrument for or study of anatomical and histological characteristics of two vegetais. To carry out the need for the sheet, brush and plant material that will be used for visualization, other than all or laboratory equipment: optical microscopy, petri dishes, sheets and laminulas, hypochlorite and corant (fuxin, lugol, among others)
Or procedure, the objective of which is to make cuts of escolhidas or thinner structures, transparent possível to allow observation of all the tenses that they form.
The main cuts are:
-Transversal:
Horizontal and total cut. Deep or enough to allow observation of two internal tecidos.
- Longitudinal:
Vertical and total cut made in the middle of vertical direction. It allows visualization of internal tecidos.
Paradermal:
Horizontal and superficial cut, allowing for observation of external tecidosis.
Or procedure for the realization of six cuts and faith in six stages:
- Initially performed or cut:
É aconselhado se fazer replicas and escort you more transparent
- Deposit or cut in petri dish with water:
Isso lets you know that the court doesn't desidrate
Deposit or cut in hypochlorite for 2 min. => Assim a clean cut. Apos must be withdrawn or excessive in water.
- Cook or cut for 2 min:
This stage is carried out with a specific corant, seja fuxin (for cores lignin) seja lugol (for cores amido). Apos must be deposited or cut to water to throw or excess of corante.
Deposit or court na lâmina;
Cover or cut with the laminula:
in position of 45º.
Após this procedure or cut is soon to be observed not microscopic optical.
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Animal tissue