BACTERIOLOGICAL CULTURE MEDIA ARE GENERALLY STERILIZED USING.. THE AUTOCLAVE

BACTERIOLOGICAL CULTURE MEDIA ARE GENERALLY STERILIZED USING:
A- THE AUTOCLAVE***
B- THE OVEN.
C- FILTERATION.
D- BUNSEN BURNER.

An autoclave is a device for sterilizing, using heat and water vapor under pressure, the equipment used in a medical environment (surgery, among others). Before being used in a medical environment, the autoclave was used for the sterilization of canned foods among others.
The autoclave consists of a heat generator, and a double-walled study. It is necessary, before sterilizing objects, to place them in waterproof boxes. Then the water is brought to boiling, which allows to obtain a moist heat of 100 to 135 ° centigrade. The duration of the autoclave is two to three quarters of an hour. A very high vacuum is then produced to obtain acceptable drying. Finally, the boxes are stored in a dry place that must remain sterile for about seven days.
Originally, an autoclave is a container whose lid is slid inside the container shell and which closes tightly under the effect of the internal pressure of the steam.
It allows to exceed the atmospheric pressure and thus to bring liquid water beyond 100 ° C. Subsequently, other types of containers allowing this action have taken this name, although their cover is only affixed to the opening and held in place by various devices.
An Advanced Surgical Antenna Sterilizer from the First World War
An autoclave is a thick-walled, hermetically sealed container designed to produce under pressure (a few bars) either an industrial reaction, or cooking or steam sterilization. For a material to be considered sterile, the theoretical probability of isolating a seed must be less than 1 in 1 million. This is the sterility assurance level (SIN) regulated by EN 556.
The principle of the autoclave was invented by Denis Papin in 1679. On April 9, 1820, Pierre-Alexandre Lemare filed a patent on the "pot autoclave" which will be improved by Nicolas Appert. His successor and successor, Raymond Chevallier-Appert, patented, on December 28, 1852, the practice of sterilization under the title "autoclave with special manometer", ancestor of the current steam sterilizer. In 1879 Charles Chamberland improved the process for medical purposes.
Autoclave is the cycle of use of an autoclave and autoclave is the verb.
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