While using the pregnancy test we are measuring?
a. B-HCG
b. Total HCG
c. B-HCG & LH
d. B-HCG & FSH.
(b) α subunit of HCG is very similar to α subunit of TSH and FSH and identical to LH. Although β subunits of HCG and LH are very similar, antibodies can be made to the β subunit of HCG that do not cross react with LH or other pituitary hormones. Most EIA use 2 monoclonal antibodies against different sites of HCG molecule one for carboxyl terminal of β chain and the other to the α chain, i.e. react with intact HCG.
a. B-HCG
b. Total HCG
c. B-HCG & LH
d. B-HCG & FSH.
(b) α subunit of HCG is very similar to α subunit of TSH and FSH and identical to LH. Although β subunits of HCG and LH are very similar, antibodies can be made to the β subunit of HCG that do not cross react with LH or other pituitary hormones. Most EIA use 2 monoclonal antibodies against different sites of HCG molecule one for carboxyl terminal of β chain and the other to the α chain, i.e. react with intact HCG.
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Clinical Chemistry